Transcription of A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch ...
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A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch ...
A Beginner s Guide to Bioprocess Modes Batch , Fed- Batch , and Continuous FermentationYing Yang and Ma ShaEppendorf Inc., Enfield, CT, USAC ontact: NOTE No. 408 AbstractIn this application note, we explain the differences between Batch , fed- Batch , and continuous fermentation and how these influence culture growth. As an example, we look at E. coli fermentation processes at bench scale. We track the biomass and nutrient concentrations during Batch , fed- Batch , and continuous fermentation runs. We explain different methods to analyze the process, including determination of biomass, growth rate, productivity, yield, and analysis of process costs. The comparisons can help Bioprocess engineers to select the most appropriate method to meet their needs. In our examples we studied E. coli fermentation at bench scale. The principles may also apply to bioprocesses using other microbes or mammalian cells, at both smaller and larger microorganisms consume carbon sources, mainly sugar, to produce various acids, alcohols, and gases.
three modes of fermentation operation. Material and Methods Bacterial strain The bacterial strain used in this study was E. coli ATCC® 25922GFP™. A mini cell bank was prepared from the cryovial obtained from ATCC, as described previously [2]. This strain has an ampicillin resistance gene (bla) encoded on a plasmid. A concentration of 0.1 g/L ...
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