Transcription of A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch, …
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A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch, …
A Beginner s Guide to Bioprocess Modes Batch, Fed-Batch, and Continuous FermentationYing Yang and Ma ShaEppendorf Inc., Enfield, CT, USAC ontact: NOTE No. 408 AbstractIn this application note, we explain the differences between batch, fed-batch, and continuous fermentation and how these influence culture growth. As an example, we look at E. coli fermentation processes at bench scale. We track the biomass and nutrient concentrations during batch, fed-batch, and continuous fermentation runs. We explain different methods to analyze the process, including determination of biomass, growth rate, productivity, yield, and analysis of process costs. The comparisons can help Bioprocess engineers to select the most appropriate method to meet their needs.
and ampicillin were also aseptically added to reach a final concentration of 90 g/L and 0.1 g/L, respectively (Table A1). For fed-batch fermentation, we used chemically defined medium as well, as described in Table A1. We prepared 1.5 L feeding medium containing MgSO 4, thiamine, trace elements, and 614 g/L glucose.
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