Transcription of A Guide to HPLC and LC-MS Buffer Selection
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A Guide to HPLC. and LC-MS . Buffer Selection HPLC Columns John Dolan Your decision has lasting effects. Choose wisely. HPLC Columns Ultra Inert Base-Deactivated HPLC Columns For Performance, Selectivity and Guaranteed Reproducibility . performance ACE guarantee If ACE does not outperform your existing column (of equivalent phase, particle size and dimensions), send in your comparative data within 60. days and keep the ACE column FREE OF CHARGE. Using Buffers with HPLC and LC-MS . Table of Contents Page Why Control pH? 2. Practical Implications 4.
phosphate and acetate can cover the entire pH range of 2<pH<8 normally used for silica-based columns. pKa (25°C) compound 0.3 trifluoroacetic acid2 2.15 phosphoric acid (pK1) 3.13 citric acid (pK1) 3.75 formic acid 4.76 acetic acid 4.76 citric acid (pK2) 4.86 propionic acid 6.35 carbonic acid (pK1) 6.40 citric acid (pK3) 7.20 phosphoric acid ...
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