Transcription of Abcam: Immunoprecipitation Protocol (IP)
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Abcam: Immunoprecipitation Protocol (IP)
Immunoprecipitation (IP) Protocol Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads. This physically isolates the protein of interest from the rest of the sample. The sample can then be separated by SDS-PAGE for Western blot analysis. 1. Lysis buffers and other reagents 2.
www.abcam.com/technical IMMUNOPRECIPITATION (IP) PROTOCOL Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of
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