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Guide to Human CD antigens

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About Abcam Abcam is a provider of protein research tools and services, with an unrivaled range of products and expert technical support, enabling scientists to analyze living cells

  Guide, Human, Antigen, Abcam, Guide to human cd antigens, Abcam abcam

ELISPOT protocol - abcam.com

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Discover more at abcam.com/technical Positive control stimulation Experiments to detect cytokines using ELISPOT will require use …

  Abcam

General protocols for inducing apoptosis in cells - abcam.com

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Discover more at abcam.com/technical 4. Harvest cells and prepare lysates for either western blotting or immunoprecipitation. For any agent used, a time course of ...

  Protocol, Western, Abcam

IHC staining protocol - Abcam

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IHC staining protocol Paraffin, frozen and free-floating sections . 2 IHC staining protocol Contents ... Most antibodies will be used in IHC-P at a concentration of 0.5–10 μg/mL. The primary antibody should be raised in a species different from the tissue being stained. Eg if you had mouse tissue and your primary antibody was raised in a

  Protocol, Paraffin, Abcam, Paraffin protocol

Protocols book - Abcam

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is also recognized by secondary antibodies in immunoprecipitation, immunoblots and immunohistochemistry. Antibodies can be cleaved into two F(ab) and one Fc

  Book, Protocol, Abcam, Protocols book

IHC-PARAFFIN PROTOCOL (IHC-P) - Abcam

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IHC-P refers to the staining of tissues that have been fixed (usually in neutral buffered formalin) and then embedded in paraffin before being sectioned. The basic steps of the IHC-P protocol are as follows: 1. Fixing and embedding the tissue 2. Cutting and mounting the section 3. Deparaffinizing and rehydrating the section 4. Antigen retrieval 5.

  Protocol, Paraffin, Abcam, Paraffin protocol, P protocol

Apoptosis - Abcam

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5 Apoptosis Apoptosis is a type of programmed cell death that is critical for numerous normal physiological processes . Historically, apoptosis has …

  Abcam, Apoptosis, Apoptosis apoptosis

Immunoprecipitation protocol - Abcam

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4 Immunoprecipitation protocol Other reagents Protease inhibitors Proteolysis, dephosphorylation and denaturation begin as soon as cells are lysed.

  Protocol, Abcam, Immunoprecipitation protocol, Immunoprecipitation, Immunoprecipitation protocol abcam

ab233473 ORAC Assay Kit - abcam.com

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ORAC Assay Kit (ab233473) is a fast and reliable kit for the direct measurement of ORAC antioxidant capacity from cell lysate, plasma, serum, tissue homogenates, and food extracts.

  Assay, Arcos, Antioxidants, Abcam, Orac assay kit, Orac antioxidant

FLUOROPHORE TABLE - Abcam

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FLUOROPHORE TABLE Dye Absorbance Wavelength Emission Wavelength Visible color Hydroxycoumarin 325 386 blue methoxycoumarin 360 410 blue Alexa fluor 345 442 blue

  Abcam

Stripping for Reprobing - Abcam

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Bring volume up to 1 L with ultrapure water. Membrane incubation Use a volume that will cover the membrane. Incubate at room temperature for 5-10 minutes. Discard buffer. 5-10 minutes fresh stripping buffer. Discard buffer. 10 minutes PBS 10 minutes PBS 5 minutes TBST 5 minutes TBST Ready for blocking stage. Harsh stripping

  Water, Abcam, Ultrapure water, Ultrapure

Direct ELISA protocol - Abcam

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Direct ELISA protocol Buffers and reagents Bicarbonate/carbonate coating buffer (100 mM) Antigen or antibody should be diluted in coating buffer to immobilize them to the wells: 3.03 g Na 2CO 3, 6.0 g NaHCO 3 1000 ml distilled water pH 9.6, PBS 1.16 g Na 2HPO 4, 0.1 g KCl, 0.1 g K 3PO 4, 4.0 g NaCl (500 ml distilled water) pH 7.4. Blocking solution

  Direct, Protocol, Abcam

Sample Preparation for Western Blot - Abcam

docs.abcam.com

2 Sample preparation for western blot Contents – Lysis buffers – Protease and phosphatase inhibitors – Preparation of lysate from cell culture – Preparation of lysate from tissues – Determination of protein concentration – Preparation of samples for loading into gels Lysis buffers Lysis buffers differ in their ability to solubilize proteins, with those containing sodium

  Western, Bolt, Abcam, Western blot

IMMUNOPRECIPITATION (IP) PROTOCOL - Abcam

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Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads.

  Purification, Protein, Abcam

Storing cells by freezing - Abcam

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Resuspend cells in enough freezing medium to create a cell suspension of 1x106 cells per ml. Pipette up and down to ensure even mixture and aliquot about 1ml into storage vials. This will provide 1x106 cells per cryovial. 5. Transfer cells immediately to -20°C for one hour, followed by -80°C overnight before permanent storage in liquid ...

  Storing, Cells, Abcam, Freezing, Storing cells by freezing

Dot blot protocol - Abcam

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Dot Blot protocol A technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.

  Protocol, Bolt, Abcam, Blot protocol

Buffers and stock solutions - Abcam

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0.5% sodium deoxycholate 0.1% SDS The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light. The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H 2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml). Store the buffer at 4°C. Nonidet-P40 (NP-40) buffer

  Sodium, Abcam

Cell culture guidelines - Abcam

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This is efficient to coat about 5 flasks. 2. Pipette coating solution into flask. Rock back and forth to evenly distribute the bottom of the flask. Let sit in incubator ... Media should be pinky orange in colour. 2. Discard cells if: • They are detaching in large numbers (attached lines) and/or look shrivelled and grainy/dark in colour. ...

  Culture, Colour, Coat, Cells, Abcam, Cell culture

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