Search results with tag "Abcam"
Guide to Human CD antigens
docs.abcam.comAbout Abcam Abcam is a provider of protein research tools and services, with an unrivaled range of products and expert technical support, enabling scientists to analyze living cells
ELISPOT protocol - abcam.com
www.abcam.comDiscover more at abcam.com/technical Positive control stimulation Experiments to detect cytokines using ELISPOT will require use …
General protocols for inducing apoptosis in cells - abcam.com
www.abcam.comDiscover more at abcam.com/technical 4. Harvest cells and prepare lysates for either western blotting or immunoprecipitation. For any agent used, a time course of ...
IHC staining protocol - Abcam
docs.abcam.comIHC staining protocol Paraffin, frozen and free-floating sections . 2 IHC staining protocol Contents ... Most antibodies will be used in IHC-P at a concentration of 0.5–10 μg/mL. The primary antibody should be raised in a species different from the tissue being stained. Eg if you had mouse tissue and your primary antibody was raised in a
Protocols book - Abcam
docs.abcam.comis also recognized by secondary antibodies in immunoprecipitation, immunoblots and immunohistochemistry. Antibodies can be cleaved into two F(ab) and one Fc
IHC-PARAFFIN PROTOCOL (IHC-P) - Abcam
www.abcam.comIHC-P refers to the staining of tissues that have been fixed (usually in neutral buffered formalin) and then embedded in paraffin before being sectioned. The basic steps of the IHC-P protocol are as follows: 1. Fixing and embedding the tissue 2. Cutting and mounting the section 3. Deparaffinizing and rehydrating the section 4. Antigen retrieval 5.
Apoptosis - Abcam
docs.abcam.com5 Apoptosis Apoptosis is a type of programmed cell death that is critical for numerous normal physiological processes . Historically, apoptosis has …
Immunoprecipitation protocol - Abcam
docs.abcam.com4 Immunoprecipitation protocol Other reagents Protease inhibitors Proteolysis, dephosphorylation and denaturation begin as soon as cells are lysed.
ab233473 ORAC Assay Kit - abcam.com
www.abcam.comORAC Assay Kit (ab233473) is a fast and reliable kit for the direct measurement of ORAC antioxidant capacity from cell lysate, plasma, serum, tissue homogenates, and food extracts.
FLUOROPHORE TABLE - Abcam
www.abcam.comFLUOROPHORE TABLE Dye Absorbance Wavelength Emission Wavelength Visible color Hydroxycoumarin 325 386 blue methoxycoumarin 360 410 blue Alexa fluor 345 442 blue
Stripping for Reprobing - Abcam
www.abcam.comBring volume up to 1 L with ultrapure water. Membrane incubation Use a volume that will cover the membrane. Incubate at room temperature for 5-10 minutes. Discard buffer. 5-10 minutes fresh stripping buffer. Discard buffer. 10 minutes PBS 10 minutes PBS 5 minutes TBST 5 minutes TBST Ready for blocking stage. Harsh stripping
Direct ELISA protocol - Abcam
www.abcam.comDirect ELISA protocol Buffers and reagents Bicarbonate/carbonate coating buffer (100 mM) Antigen or antibody should be diluted in coating buffer to immobilize them to the wells: 3.03 g Na 2CO 3, 6.0 g NaHCO 3 1000 ml distilled water pH 9.6, PBS 1.16 g Na 2HPO 4, 0.1 g KCl, 0.1 g K 3PO 4, 4.0 g NaCl (500 ml distilled water) pH 7.4. Blocking solution
Sample Preparation for Western Blot - Abcam
docs.abcam.com2 Sample preparation for western blot Contents – Lysis buffers – Protease and phosphatase inhibitors – Preparation of lysate from cell culture – Preparation of lysate from tissues – Determination of protein concentration – Preparation of samples for loading into gels Lysis buffers Lysis buffers differ in their ability to solubilize proteins, with those containing sodium
IMMUNOPRECIPITATION (IP) PROTOCOL - Abcam
www.abcam.comImmunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads.
Storing cells by freezing - Abcam
www.abcam.comResuspend cells in enough freezing medium to create a cell suspension of 1x106 cells per ml. Pipette up and down to ensure even mixture and aliquot about 1ml into storage vials. This will provide 1x106 cells per cryovial. 5. Transfer cells immediately to -20°C for one hour, followed by -80°C overnight before permanent storage in liquid ...
Dot blot protocol - Abcam
www.abcam.comDot Blot protocol A technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.
Buffers and stock solutions - Abcam
www.abcam.com0.5% sodium deoxycholate 0.1% SDS The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light. The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H 2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml). Store the buffer at 4°C. Nonidet-P40 (NP-40) buffer
Cell culture guidelines - Abcam
www.abcam.comThis is efficient to coat about 5 flasks. 2. Pipette coating solution into flask. Rock back and forth to evenly distribute the bottom of the flask. Let sit in incubator ... Media should be pinky orange in colour. 2. Discard cells if: • They are detaching in large numbers (attached lines) and/or look shrivelled and grainy/dark in colour. ...
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