Transcription of Immunoprecipitation protocol - Abcam
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Immunoprecipitation protocol - Abcam
Immunoprecipitation protocol General Immunoprecipitation procedure and required reagents 2 Immunoprecipitation protocol Contents Lysis buffers Other reagents Preparing the lysates Pre-clearing the lysates Immunoprecipitation Washing Elution Choosing the correct beads summary table References Lysis buffers The ideal lysis buffer will minimize protein denaturation while releasing an adequate amount of proteins from the sample. Non-ionic detergents such as NP-40 and Triton X-100 are less harsh than ionic detergents such as SDS and sodium deoxycholate.
4 Immunoprecipitation protocol Other reagents Protease inhibitors Proteolysis, dephosphorylation and denaturation begin as soon as cells are lysed.
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