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Discover more at abcam.com/technical 4. Harvest cells and prepare lysates for either western blotting or immunoprecipitation. For any agent used, a time course of ...

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2 Immunoprecipitation protocol Contents – Lysis buffers – Other reagents – Preparing the lysates – Pre-clearing the lysates – Immunoprecipitation – Washing – Elution – Choosing the correct beads –summary table – References Lysis buffers The ideal lysis buffer will minimize protein denaturation while releasing an adequate

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30. Chenderen K, Gao Y, Campbell YG, Steve Smith and Patterson SZ. The relationships among nm23 gene expression, tumor genesis and metastasis in human salivary gland tumor.

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