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Blot Protocol

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Dot blot protocol - Abcam

www.abcam.com

Dot Blot protocol A technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.

  Protocol, Bolt, Abcam, Blot protocol

CRISPR / Cas9 KO Plasmid PROTOCOL and HDR Plasmid ...

datasheets.scbt.com

This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. • 5In a 6-well tissue culture plate seed 1.5 x 10 - 2.5 x 105 cells in 3 ml of antibiotic-free standard growth medium per well, 24 hours prior to transfec-tion.

  Protocol

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