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Found 2 free book(s)Sample Preparation for Western Blot - Abcam
docs.abcam.com2 Sample preparation for western blot Contents – Lysis buffers – Protease and phosphatase inhibitors – Preparation of lysate from cell culture – Preparation of lysate from tissues – Determination of protein concentration – Preparation of samples for loading into gels Lysis buffers Lysis buffers differ in their ability to solubilize proteins, with those containing sodium
Abcam: Immunoprecipitation Protocol (IP)
www.abcam.comwww.abcam.com/technical IMMUNOPRECIPITATION (IP) PROTOCOL Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of