Transcription of IMMUNOPRECIPITATION (IP) PROTOCOL - Abcam
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IMMUNOPRECIPITATION (IP) PROTOCOL - Abcam
IMMUNOPRECIPITATION (IP) PROTOCOL IMMUNOPRECIPITATION is a method that enables the purification of a protein . An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads. This physically isolates the protein of interest from the rest of the sample. The sample can then be separated by SDS-PAGE for Western blot analysis. 1. Lysis buffers and other reagents 2.
Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads.
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