Transcription of Protocols book - Abcam
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Protocols book 2017/18. Contents Welcome to Abcam About us.. 5. Extensive validation.. 5. Comprehensive information .. 5. 100% support.. 5. Fast global delivery.. 6. Abreviews .. 6. Sign up for an Abcam account .. 6. Protocols Section1: Antibodies and antibody structure.. 7. Section 2: Formats of antibody and antibody purification.. 11. Section 3: Choosing an antibody and antibody dilution .. 13. Section 4: Fluorescence .. 16. How single fluorochromes work.. 16. Fluorochrome table (excitation and emission wavelengths) .. 18. Section 5: Western blot .. 19. Sample preparation .. 22. Lysis buffers.. 22. Protease and phosphatase inhibitors.
is also recognized by secondary antibodies in immunoprecipitation, immunoblots and immunohistochemistry. Antibodies can be cleaved into two F(ab) and one Fc
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