Transcription of IHC staining protocol - Abcam
{{id}} {{{paragraph}}}
Example: confidence
IHC staining protocol - Abcam
IHC staining protocol paraffin , frozen and free-floating sections IHC staining protocol Contents paraffin and frozen sections Immunostaining free-floating sections Signal amplification paraffin and frozen sections Reagents can be applied manually by pipette, or the protocol can be adapted for automated and semi-automated systems if these are available. Carry out incubations in a humidified chamber to avoid tissue drying out, which will lead to non-specific binding and high background staining . A shallow plastic box with a sealed lid and wet tissue paper in the bottom is an adequate chamber. Keep slides off the paper and lay flat so that the reagents don't drain off.
IHC staining protocol Paraffin, frozen and free-floating sections . 2 IHC staining protocol Contents ... Most antibodies will be used in IHC-P at a concentration of 0.5–10 μg/mL. The primary antibody should be raised in a species different from the tissue being stained. Eg if you had mouse tissue and your primary antibody was raised in a
Loading..
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: