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IHC staining protocol - Abcam
IHC staining protocol Paraffin, frozen and free-floating sections . 2 IHC staining protocol Contents ... Most antibodies will be used in IHC-P at a concentration of 0.5–10 μg/mL. The primary antibody should be raised in a species different from the tissue being stained. Eg if you had mouse tissue and your primary antibody was raised in a
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IHC-PARAFFIN PROTOCOL (IHC-P) - Abcam
www.abcam.comIHC-P refers to the staining of tissues that have been fixed (usually in neutral buffered formalin) and then embedded in paraffin before being sectioned. The basic steps of the IHC-P protocol are as follows: 1. Fixing and embedding the tissue 2. Cutting and mounting the section 3. Deparaffinizing and rehydrating the section 4. Antigen retrieval 5.