Search results with tag "Immunoprecipitation"
萤火素酶互补实验检测蛋白互作
www.chinbullbotany.com疫共沉淀(Co-Immunoprecipitation, Co-IP) 、双分子 荧光互补(Bimolecular Fluorescence Compleme-ntation, BiFC)及荧光共振能量转移技术(Fluores-cence Resonance Energy Transfer, FRET)等 (Morsy et al., 2008; 徐重益, 2020)。不同的蛋白互 作研究技术各有优缺点。酵母双杂交 …
Buffers and stock solutions for western blot
docs.abcam.comimmunoprecipitation and pull-down assays). – 50 mM Tris HCl, pH 8.0 – 150 mM NaCl – 1% NP-40 – 0.5% sodium deoxycholate – 0.1% SDS The 10% sodium deoxycholate stock solution (5 g into 50 mL) must be protected from light. NP-40 buffer – 20 mM Tris HCl – pH 8.0 137 mM NaCl – 10% glycerol – 1% NP-40 – 2 mM EDTA
Co-immunoprecipitation (co-IP) Troubleshooting Guide
www.proteinguru.comTECH TIP 1 Thermo Fisher Scientific Industrielaan 27 +32 53 83 44 04 www.thermo.com/perbio Industriezone III 9320 Erembodegem, Belgium +32 53 83 76 38 fax
doi: 10.1007/978-1-4939-7380-4 18 - muehlemann.dcb.unibe.ch
www.muehlemann.dcb.unibe.chChapter 18 Spike-In Normalization of ChIP Data Using DNA–DIG–Antibody Complex Andrea B. Eberle Abstract Chromatin immunoprecipitation (ChIP) is a widely used …
クロマチン免疫沈降法プロトコール
www.cosmobio.co.jp1 / 15 クロマチン免疫沈降法プロトコール (ChIP Assay : Chromatin Immunoprecipitation Assay) 【目次】
General protocols for inducing apoptosis in cells - abcam.com
www.abcam.comDiscover more at abcam.com/technical 4. Harvest cells and prepare lysates for either western blotting or immunoprecipitation. For any agent used, a time course of ...
Immunoprecipitation (IP) - Thermo Fisher Scientific
tools.thermofisher.comImmunoprecipitation (IP) and co-immunoprecipitation (Co-IP) are methods used to enrich or purify a specific protein or group of proteins from a complex mixture using an antibody immobilized on a solid support. IP is an important step in many
Immunoprecipitation protocol - Abcam
docs.abcam.com6 Immunoprecipitation protocol Pre-clearing the lysates Pre-clearing the lysate can help reduce non-specific binding and reduce background. However, if the final detection of …