Search results with tag "Fluorochrome"
ANILINE BLUE FLUOROCHROME - Biosupplies
www.biosupplies.com.auCHEMICAL PROPERTIES Aniline blue fluorochrome is a chemically synthesized fluorochrome identical with the component of commercial aniline blue (CI
A Guide to Fluorochromes
docs.abcam.comFluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. A tandem dye consists of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two.
A Guide to Fluorochromes - Abcam
docs.abcam.comA Guide to Fluorochromes Discover more at abcam.com/fluorochromes 1 4 8 _ 1 2 _ A K R Copyright © 2012 Abcam, All Rights Reserved. The Abcam logo is a registered ...
BD FACSCanto II
www.bd.comEmission spectra of commonly used fluorochromes The BD FACSCanto II system is designed for these fluorophore combinations. Excitation optics focus the laser beams Instrument Laser Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSCanto II flow cytometry system Solid State (L1) 488Green FITC Alexa ...
Immunocytochemistry (ICC)
images.novusbio.comThe brightness of fluorochromes is directly proportional to the extinction coefficient, a measurement of the fluorophore’s probability of absorbing a photon of light. For example, a dim fluorochrome, such as DyLightTM 350, has ε value = 15,000, whereas a brighter member of this family, DyLightTM 650, has an ε value = 250,000.
BD Biosciences Relative Fluorochrome Brightness
www.bu.eduThis table provides general guidance with respect to the relative capability of different fluorochromes to resolve dimly stained populations; it is not a representation of absolute fluorescence. Rankings were determined by comparing the stain index (reso lution) of cells stained with multiple formats on several clones run on a variety of flow ...
BD バイオサイエンス 蛍光色素表
www.bdj.co.jpInstrument Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences Accuri® C6 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE PI FL3 Red 7-AAD PerCP PerCP-Cy™5.5 PE-Cy™7 640 FL4 Red APC Alexa Fluor® 647 BD FACSCalibur™ 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE PI
Immunohistochemistry (IHC)
images.novusbio.comFluorochromes (numerous options) Chromogenic Reagents: Enzymes (HRP, AP) and Substrates (DAB, AEC, NBT/BCIP) Counterstain Fluorescence: DAPI, Hoechst 33342 Chromogenic: Hematoxylin, Fast Green FCF *The above table is not intended to be exhaustive but rather summarizes common factors influencing IHC experimental design at Bio-Techne.
MGITTM Procedure Manual - FIND
www.finddx.orgfluorochrome, tris 4, 7-diphenyl-1, 10-phenonthroline ruthenium chloride pentahydrate, embedded in silicone at the bottom of the tube. During bacterial growth within the tube, the free oxygen is utilized and is replaced with carbon dioxide. With depletion of free oxygen,
Fluorescence Excitation and Emission Fundamentals
www.chem.uci.eduFluorochromes that are conjugated to a larger macromolecule (such as a nucleic acid, lipid, enzyme, or protein) through adsorption or covalent bonds are termed fluorophores. In general, fluorophores are divided into two broad classes, termed intrinsic and extrinsic. Intrinsic fluorophores, such as
18 Focus PCR et ARN 16S Mise en page 1 - Eurofins Biomnis
www.eurofins-biomnis.comddNTP sont incorporés, marqués par 4 fluorochromes différents. Les fragments d’ADN obtenus sont de longueurs différentes ; le séquenceur sépare les fragments d’ADN selon leur taille par chromatographie. Les systèmes actuels détectent la florescence des 4 nucléotides à partir de la même colonne. Le résultat est
Red Blood Cell (RBC) Lysis Protocols
tools.thermofisher.comAll of the RBC lysis reagents are compatible with fluorochrome-conjugated antibodies. Protocol A: Using 1X or 10X RBC Lysis Buffers Both the 1X and 10X RBC Buffers are designed to lyse RBC in whole blood (using heparin or EDTA as the anti-coagulant) or tissue preparations using ammonium chloride-based osmotic shock. The 10X RBC
for FLUORESCENCE MICROSCOPY - Chroma
www.chroma.comThis guide is a compilation of the principles and know-how that the engineers ... fluorochromes, and when conjugated to other organically active substances, such as antibodies and nucleic acids, they are called fluorescent probes or fluorophores. (These various terms are often used interchangeably.) There
BD Horizon™ Brilliant Violet™ 510 Reagents
www.bdbiosciences.comTitle: BD Horizon™ Brilliant Violet™ 510 Reagents Subject: BV510, a new dye for the violet laser, is brighter than currently available fluorochromes in the V500 detector.
PRINCIPALES TECHNIQUES DE BIOLOGIE MOLECULAIRE ET …
mediprepa.orgutilisée. Chaque ddNTP a un fluorochrome différent. B. Les RFLP (Rrestriction Fragment Lenght Polymorphism). Cette technique permet de détecter une mutation qui crée ou abolit un site de restriction et qui est associée à une pathologie. 1. Amplification par PCR de la séquence qui contient le site de restriction. 2.
Bedinger Lab Tomato: Pollen Staining Protocols
www.irbtomato.orga. Aniline Blue Fluorochrome was obtained from Biosupplies Australia (http://www.biosupplies.com.au). We prep a stock solution of 0.1mg/ml in ddH2O from the
Techniques de biologie moléculaire, génie génétique et ...
www.svt-tanguy-jean.comfluorochromes . • La révélation par fluorescence permet de savoir si les fragments d’ADN déposés se sont ou non hybridés avec les sondes de la puce , et donc si telle ou telle . 1. 2.
Fluorochromes for Flow Cytometry and Microscopy
www.biolegend.comFluorochromes for Flow Cytometry and Microscopy Fluorochrome Excitation Max (nm) Excitation Laser Lines (nm) Emission Max (nm) BD FAC-Scan™ BD FAC-
Fluorochrome/Laser Reference Poster - Choose Region
www.bdbiosciences.comFor Research Use Only. Not for use in diagnostic or therapeutic procedures. APC-Cy7: US patent 5,714,386 Cy™ is a trademark of GE Healthcare. Cy dyes are subject to ...
Fluorochrome/Laser Reference Poster - BD Biosciences
www.bdbiosciences.comFor Research Use Only. Not for use in diagnostic or therapeutic procedures. APC-Cy7: US patent 5,714,386 Cy™ is a trademark of GE Healthcare. Cy dyes are subject to ...
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Aniline Blue Fluorochrome, A Guide to Fluorochromes, Fluorochromes, Emission, Immunocytochemistry ICC, BD Biosciences Relative Fluorochrome Brightness, Procedure Manual, Fluorochrome, Guide, Brilliant Violet, PRINCIPALES TECHNIQUES DE BIOLOGIE MOLECULAIRE, Bedinger Lab Tomato: Pollen Staining Protocols, Biosupplies, Techniques de biologie moléculaire, génie, Fluorochromes for Flow Cytometry and Microscopy, Fluorochrome/Laser Reference Poster, Fluorochrome/Laser