A Guide To Fluorochromes
Found 9 free book(s)
A Guide to Fluorochromes
docs.abcam.comFluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. A tandem dye consists of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two.
Immunocytochemistry (ICC)
images.novusbio.comthe filter name. In addition, a rough guide for laser/filter selection is provided by the number listed after some dyes, such as 488 in Alexa Fluor® 488 or DyLightTM 488. Extinction coefficients (ε) and brightness of fluorochrome The brightness of fluorochromes is directly proportional to the extinction coefficient,
for FLUORESCENCE MICROSCOPY - Chroma
www.chroma.comThis guide is a compilation of the principles and know-how that the engineers ... fluorochromes, and when conjugated to other organically active substances, such as antibodies and nucleic acids, they are called fluorescent probes or fluorophores. (These various terms are often used interchangeably.) There
Fluorophore selection guide for flow cytometry
cytobase.montp.inserm.frFluorophore selection guide Dyes for the 405 nm and 407 nm lasers Pacific Blue™ → The dye of choice for the first violet channel → Excited optimally at ~410 nm with emission maximum at ~455 nm → No UV excitation → Minimal spectral overlap with green fluorophores Alexa Fluor® 405
BD FACSCanto II
www.bd.comEmission spectra of commonly used fluorochromes The BD FACSCanto II system is designed for these fluorophore combinations. Excitation optics focus the laser beams Instrument Laser Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSCanto II flow cytometry system Solid State (L1) 488Green FITC Alexa ...
Immunohistochemistry (IHC)
images.novusbio.comFluorochromes (numerous options) Chromogenic Reagents: Enzymes (HRP, AP) and Substrates (DAB, AEC, NBT/BCIP) Counterstain Fluorescence: DAPI, Hoechst 33342 Chromogenic: Hematoxylin, Fast Green FCF *The above table is not intended to be exhaustive but rather summarizes common factors influencing IHC experimental design at Bio-Techne.
BD バイオサイエンス 蛍光色素表
www.bdj.co.jpInstrument Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences Accuri® C6 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE PI FL3 Red 7-AAD PerCP PerCP-Cy™5.5 PE-Cy™7 640 FL4 Red APC Alexa Fluor® 647 BD FACSCalibur™ 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE PI
フローサイトメトリー - Bio-Rad
www.bio-rad.comはじめに 本書はBio-Rad Laboratories 社発行の”Flow Cytometry Basic Guide”の日本語翻訳版です。 より理解を深めるため本書には原本にない章や記載が日本語版のみで追加されています。
BD Biosciences Relative Fluorochrome Brightness
www.bu.eduThis table provides general guidance with respect to the relative capability of different fluorochromes to resolve dimly stained populations; it is not a representation of absolute fluorescence. Rankings were determined by comparing the stain index (reso lution) of cells stained with multiple formats on several clones run on a variety of flow ...