Transcription of Amphibian Metamorphosis Assay OCSPP Guideline 890
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Amphibian Metamorphosis Assay OCSPP Guideline 890
Amphibian Metamorphosis Assay OCSPP Guideline Standard Evaluation Procedure (SEP). ENDOCRINE DISRUPTOR SCREENING PROGRAM. Environmental Protection Agency Washington, DC 20460. 30 September 2011. Standard Evaluation Procedure (SEP). Amphibian Metamorphosis Assay , OCSPP Table of Contents I. 1. II. THE Amphibian Metamorphosis Assay .. 2. A. Purpose of the Assay .. 2. B. Study Design .. 2. III. EVALUATION OF STUDY CONDUCT .. 2. A. Test Species .. 3. B. Equipment and Supplies .. 3. C. Chemical Testability .. 3. D. Exposure System .. 3. 1. System Description .. 3. 2. Water Quality .. 3. 3. Iodide Concentration in Test 4. E. Holding of Animals .. 4. 1. Adult Care and Breeding .. 4. 2. Embryo Selection .. 4. 3. Larval Culture and Feeding .. 4. F. Analytical 5. G. Selection of Test 5. 1. Establishing the High Test Concentration .. 5.
important assay because amphibian metamorphosis provides a well-studied, thyroid- dependent process which responds to substances known to be active along the HPT axis, and it is the only in the EDSP Tier 1 battery assay that detects thyroid activity in an
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