Analysis and Isolation of Adipocytes by Flow …

CHAPTER FIFTEENA nalysis and Isolation ofAdipocytes by Flow CytometrySusan M. Majka*, Heidi L. Miller , Karen M. Helm{, Alistaire S. Acosta{,Christine R. Childs{, Raymond Kong}, Dwight J. Klemm ,1*Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA{Cancer Center Flow cytometry Core, University of Colorado Anschutz Medical Campus, Aurora, Colorado,USA}Amnis Corporation, Seattle, Washington, USA1 Corresponding author: e-mail address: of Adipocytes by Collagenase Digestion of Adipose of Single Cell Suspensions from Adipose Tissue for Flow Deep Red neutral lipid staining of lipid antibody staining to exclude stromal/vascular cells Violet staining to identify events with nuclei and distinguishsinglets from and Sorting of Adipocytes by Flow XDP of Adipocytes from stromal cells based on cell size: FSC versus of single cells (singlets) from cell of events containing lipid of stromal/vascular of adipocyt}}

CHAPTER FIFTEEN Analysis and Isolation of Adipocytes by Flow Cytometry SusanM.Majka*,HeidiL.Miller†,KarenM.Helm{,AlistaireS.Acosta{, Christine R. Childs{, Raymond Kong}, Dwight J. Klemm†,1

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  Analysis, Isolation, Flows, Adipocyte, Analysis and isolation of adipocytes by flow, Analysis and isolation of adipocytes by flow cytometry, Cytometry

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