Transcription of AnintroductiontoNext-Generation Sequencing Technology
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AnintroductiontoNext-Generation Sequencing Technology
An introduction to Next-Generation Sequencing Technology For Research Use Only. Not for use in diagnostic procedures. Table of Contents Table of Contents 2. I. Welcome to Next-Generation Sequencing 3. a. The Evolution of Genomic Science 3. b. The Basics of NGS Chemistry 4. c. Advances in Sequencing Technology 5. Paired-End Sequencing 5. Tunable Coverage and Unlimited Dynamic Range 6. Advances in Library Preparation 6. Multiplexing 7. Flexible, Scalable Instrumentation 7. II. NGS Methods 8. a. Genomics 8. Whole-Genome Sequencing 8. Exome Sequencing 8.
Figure 2:HumanGenomeSequencingOvertheDecades—Thecapacitytosequenceall3.2billionbasesofthehumangenome(at30×coverage)hasincreased exponentiallysincethe1990s.In2005 ...
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