Transcription of Apoptosis - Abcam
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Apoptosis - Abcam
ApoptosisTools for cell death series 13 ContentsCell death ..4 Apoptosis ..5 Mechanisms of Apoptosis ..5 Hallmarks of Apoptosis ..7 Detecting Apoptosis .. 8 Activation of pro-apoptotic members of the Bcl-2 family ..8 Loss of membrane asymmetry ..9 Caspases ..11 Antibody-based methods ..12 Substrate-based methods ..13 Calpain and cathepsins ..14 Mitochondrial transmembrane potential ..15 Cytochrome c release ..17 Chromatin condensation ..19 Genomic DNA fragmentation ..19 Increase of sub G1 population ..21 Cell membrane blebbing ..22 Tips for Apoptosis assays ..23 Use of Apoptosis inducers and inhibitors ..23 Analyze single cells ..23 Be aware of cell line variation ..24 General considerations ..25 References ..274 Cell deathCell death happens when a cell fails to maintain essential life functions and can be non-programmed, in the case of injury or trauma, or programmed, as in processes like Apoptosis and autophagy .Cell death can be classified according to its morphological appearance (such as apoptotic or necrotic), enzymological criteria (with or without the involvement of distinct proteases), functional aspects (programmed or non-programmed), or immunological characteristics (immunogenic or non-immunogenic)1.
5 Apoptosis Apoptosis is a type of programmed cell death that is critical for numerous normal physiological processes . Historically, apoptosis has been defined by its morphological
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