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Application of Inter Simple Sequence Repeat (ISSR) Marker ...

Kasetsart J. (Nat. Sci.) 43 : 339 - 347 (2009) Application of Inter Simple Sequence Repeat (ISSR) Markerfor Typing of saccharomycopsis fibuligera Isolatedfrom Loog-pang, Kao-mag and SathoSaowalak Dansakul1*, Vichein Leelawatcharamas1,Charoen Charoenchai2 and Hathairat Urairong3 ABSTRACTThe D1/D2 26S rDNA Sequence was shown to be applicable for yeast species size of this region was 600 bp and the identification results showed more than 98% similarity of allisolates compared to the sequences in the Genbank database. This research also included a small surveyfor ISSR variation in the yeast saccharomycopsis fibuligera . Eight of twelve UBC (University of BritishColumbia) primers gave 80 reproducible patterns. The size of the ISSR products ranged from 300 toabout 2000 bp.

Key words: Saccharomycopsis fibuligera, ISSR (inter simple sequence repeat), UBC primers, loog- pang, kao-mag, satho 1 Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.

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  Simple, Sequence, Inter, Parete, Inter simple sequence repeat, Saccharomycopsis fibuligera, Saccharomycopsis, Fibuligera

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