Application of Inter Simple Sequence Repeat (ISSR) Marker ...

Key words: Saccharomycopsis fibuligera, ISSR (inter simple sequence repeat), UBC primers, loog- pang, kao-mag, satho 1 Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.

  Simple, Sequence, Inter, Parete, Inter simple sequence repeat, Saccharomycopsis fibuligera, Saccharomycopsis, Fibuligera

Novel method to establish molecular identity using inter ...

The inter simple sequence repeat (ISSR) technique is another PCR-based method, which involves amplification of the DNA segments present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite directions.

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Khaled Elmeer, Ameena Almalki

suggested that ISSR (Inter Simple Sequence Repeat) markers are the best suited DNA markers for genetic variation analysis in tree species like . P. cineraria. ISSR-PCR is a technique, which involves the use of microsatellite sequences as primers in a polymerase

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Molecular analysis of genetic fidelity in Cannabis sativa L ...

L. plants grown from synthetic (encapsulated) seeds ... stability of synthetic seeds of Cannabis sativa L. during in vitro multiplication and storage for 6 months at different growth conditions using inter simple sequence repeat (ISSR) DNA fingerprinting.

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Genetic Diversity of Cinnamomum porrectum (Roxb.) Kosterm ...

Inter simple sequence repeat (ISSR) markers were used to analyze the genetic diversity of six populations in Phangnga, Phuket, Phatthalung, Satun, Krabi, and Songkhla provinces.

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GENETIC VARIATION AMONG PHYTOPHTHORA INFESTANS …

pak. j. bot., 39(3): 897-902, 2007. genetic variation among phytophthora infestans (tomato blight) isolates from western turkey revealed by inter simple sequence repeat (issr) …

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EVALUATION OF INTER-SIMPLE SEQUENCE REPEAT (ISSR) …

Inter-simple sequence repeat (ISSR) markers were evaluated for mapping onto the genetic linkage map of an oil palm interspecific hybrid mapping population (Elaeis oleifera × E. guineensis). The genetic linkage of the markers was determined using the two-way …

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THE UTILITY OF INTER-SIMPLE SEQUENCE REPEAT …

Determine if the inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) DNA fingerprinting method was suitable or sensitive enough to detect genetic variation and distinguish geographic populations of the smoke-tree sharpshooter (Homalodisca liturata) and 2.

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*Corresponding author: hamzapalmier@yahoo - cropj.com

Abbreviations: analysis of molecular variance (AMOVA), inter-simple sequence repeat (ISSR), simple sequence repeat (SSR), random amplified polymorphic DNA (RAPD), principle component analysis (PCA), random amplified microsatellites polymorphism

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Molecular Characterization of Beauvaria sp - sphinxsai.com

The inter-simple sequence repeat (ISSR) is a new kind of molecular marker that amplifies inter- microsatellite sequences at multiple loci throughout the genome …

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THE UTILITY OF THE INTERNAL TRANSCRIBED SPACER …

The utility of inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) to distinguish geographic populations of the smoketree sharpshooter Homalodisca liturata and egg parasitoids of the genus Gonatocerus , pp. 298-301.

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Genetic relationship in Coffea species and parentage ...

Genetic relationship in Coffeaspecies and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers Paulo M. Ruas 1 , Claudete F. Ruas 1 , Leandro Rampim 1 , Valdemar P. Carvalho 1 , Eduardo A. Ruas 1

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Journal of Biodiversity and Environmental Sciences (JBES)

Inter-simple sequence repeats (ISSRs) involve the use of microsatellite sequences directly in the polymerase chain reaction (PCR) for DNA amplifications (Gupta et al. 1994; Zietkiewicz et al. 1994) which combines most of the benefits of AFLP and microsatellite …

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A new intervarietal linkage map and its application for ...

inter-simple sequence repeat markers. Out of 570 loci genotyped, 330 were chosen based on their positions on the link- age map to create a “framework” map for quantitative trait locus (QTL) analysis.

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A Simple Method for Developing Microsatellite Markers ...

A Method for Isolating Microsatellites 383 Results and Discussion As a first step in the present study, we amplified inter- simple sequence repeat fragments using DNA templates

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Assessment of the Genetic Stability of Micropropagated ...

Inter-simple sequence repeat (ISSR) markers were used to evalu- ate the genetic stability of the micropropagated plants of Canna- bis sativa over 30 passages in culture and hardening in soil for 8

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Analysis of genetic relationships and identification of ...

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software

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11 2010. - Montana State Univ

11 2010. Keith B, Brummer T, Dyer W, Maxwell B, and Rew LJ Deciphering dispersal patterns of Dalmatian toadflax. ... Inter-simple sequence repeat (ISSR) analysis was employed to develop phylogenetic maps of populations. Twenty ISSR primers we re analyzed for the

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APPENDIX : MOLECULAR SYSTEMATICS OF SHRIMPS USING …

98 CHAPTER – 3 APPENDIX : MOLECULAR SYSTEMATICS OF SHRIMPS USING INTER-SIMPLE SEQUENCE REPEAT (ISSR) Introduction The ISSR marker system …

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Development of ISSR PCR markers for diversity study in ...

genetic diversity using 19 Inter-Simple Sequence Repeats (ISSR) primer pairs. Out of 22 genotypes analyzed, 14 were of C. florida , two of C. mas , one of C. nutalli , two of C. kousa, and

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Genetic Diversity of Vitex negundo L. (Lagundi) in the ...

Genetic Diversity of Vitex negundo L. (Lagundi) in the Philippines using Inter-simple Sequence Repeat (ISRR) Marker Analysis Nonnatus S. Bautista1*, Anna Francesca Cubos 2 and Maria Catherine Lim 1Plant Biology Division, Institute of Biological Sciences, University of the Philippines Los Baños, College, Laguna 4031 Philippines

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Species clarification of Isaria isolates used as ...

Finally, the inter-simple sequence repeat (ISSR) genotyping method revealed that the CHE-CNRCB 303, 305, and 307 isolates belong to three different genotypes. This result indicates that ISSR markers could be used as a tool to monitor their presence in field conditions.

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Inter simple sequence repeat (ISSR) polymorphism and its ...

Inter simple sequence repeat (ISSR)-PCR is a technique, which involves the use of microsatellite sequences as primers in a polymerase chain reaction to generate multilocus markers. It is a simple ...

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Inter Simple Sequence Repeat (ISSR) Markers for Genetic ...

Principles of Inter Simple Sequence Repeat (ISSR) markers ISSR involves amplification of DNA segments present at an amplifiable distance in between two identical microsatellite repeat regions ...

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Inter Simple Sequence Repeat Fingerprints for Assess ...

Inter Simple Sequence Repeat Fingerprints for Assess Genetic Diversity of Tunisian Garlic Populations Submitted by Emmanuel Lemoine on Thu, 02/12/2015 - 13:03

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Inter-Simple Sequence Repeat (ISSR) Markers: Are We Doing ...

Inter-simple sequence repeats (ISSRs) are regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can

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International Journal of Current Research in - ijcrbp.com

Mar 11, 2016 · China Using Inter-simple Sequence Repeat Markers Jinping Wu, Zili Ding, Zhenbiao Jiao, Leifu Chen, Zhengming Qiu and Fengling Guo* Hubei Academy of Agricultural Sciences, Wuhan 430064, China *Corresponding author. Abstract Art icle Inf o

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Inter-simple-sequence-repeat (ISSR) polymorphisms are ...

Table1 Segregation of ISSR fragments in chickpea RILs developed from a cross of ICC-4958 and PI 489777 Primer no. Sequence! Fragment s2 P size (bp) (1:1)

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Genetic diversity and differentiation of Lepidoglyphus ...

Genetic diversity and differentiation of Lepidoglyphus ...