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THE UTILITY OF INTER-SIMPLE SEQUENCE REPEAT …

- 298 - THE UTILITY OF INTER-SIMPLE SEQUENCE REPEAT -POLYMERASE CHAIN REACTION (ISSR-PCR) TO DISTINGUISH GEOGRAPHIC POPULATIONS OF THE SMOKE-TREE SHARPSHOOTER AND EGG PARASITOID SPECIES OF THE GENUS GONATOCERUS Project Leader: Jesse H. de Le n USDA, ARS Beneficial Insect Research Unit Weslaco, TX 78596 Cooperators: James R. Hagler USDA, ARS Western Cotton Res. Lab. Phoenix, AZ 85040 Guillermo Logarzo USDA, ARS South American Biological Control Lab. Buenos Aires, Argentina David J. W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501 Reporting Period: The results reported here are from work conducted fiscal year 2004 to fiscal year 2005. ABSTRACT In the current study, we tested the UTILITY of the INTER-SIMPLE SEQUENCE REPEAT -polymerase chain reaction (ISSR-PCR) DNA fingerprinting method in distinguishing geographic populations of the smoke-tree sharpshooter (STSS) (Homalodisca liturata Ball) and in distinguishing several egg parasitoid species in the genus Gonatocerus.

Determine if the inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) DNA fingerprinting method was suitable or sensitive enough to detect genetic variation and distinguish geographic populations of the smoke-tree sharpshooter (Homalodisca liturata) and 2.

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  Simple, Sequence, Inter, Parete, Inter simple sequence repeat

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