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BASIC CULTURE TECHNIQUE STREAK PLATE

Copyright Chester R. Cooper, Jr. 2022 Microbiology Laboratory (BIOL 3702L) Page 1 of 6 BASIC CULTURE TECHNIQUE : STREAK PLATE Principle and Purpose The isolation of pure cultures of microorganisms is a TECHNIQUE essential to many types of experiments in microbiology as well as in the identification of potential pathogens. One very common way to isolate bacterial and other microbes is by employing the STREAK PLATE TECHNIQUE . In essence, the STREAK PLATE TECHNIQUE is a type of dilution scheme in which a single colony presumably arises from a single cell. In practice, a gradient of cells is established on the surface of an agar PLATE .

The exact streak plate method that one uses to generate isolated colonies is not too critical. What is critical is the generation of isolated colonies. However, to isolate colonies, some folks prefer the “three-phase” streak plate technique, whereas others prefer the “four-quadrant” (four-phase) technique.

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