PDF4PRO ⚡AMP

Modern search engine that looking for books and documents around the web

Example: dental hygienist

GELATIN HYDROLYSIS TEST

Copyright 2019 by Chester R. Cooper, Jr. Microbiology Laboratory (BIOL 3702L) Page 1 of 3 GELATIN HYDROLYSIS TEST Principle and Purpose GELATIN is a soluble mixture of polypeptides derived from the collagen of animal connective tissues. A common dessert beloved by children is Jello, which is comprised of GELATIN ( ; Hence, vegans or vegetarian might think twice before eating that fruit salad Jello!) At warmer temperatures, GELATIN exists in a semi-liquidized state. At cooler temperatures, however, the polypeptide nature of GELATIN causes it to be semi-solid. Again, when making Jello, the heated, liquid mixture is placed into a mold (perhaps with fruit chunks), then place the mold in the refrigerator to cause the Jello to solidify and, viol , a tasty, refreshing dessert is formed! In the microbiology laboratory, GELATIN can be used to not only help identify bacteria, but also to assess their potential pathogenicity.

2) Using aseptic technique and a microbiology needle, inoculate the respective tube with the appropriate bacterium by stabbing the medium three-fourths of the way to the bottom of the tube. 3) Incubate all tubes for 1 to 7 days at 37°C. 4) Periodically throughout the incubation period, remove the tubes from the incubator and

Fullscreen Download
Loading..

Tags:

  Technique, Aseptic, Incubation, Aseptic technique

Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Spam in document Broken preview Other abuse

Transcription of GELATIN HYDROLYSIS TEST

Documents from same domain

BASIC CULTURE TECHNIQUE STREAK PLATE

crcooper01.people.ysu.edu

3) Using aseptic technique, sterilize a microbiological loop using either the gas burner or the Bacti-Cinerator (see the exercise entitled Basic Microbiology Technique: Aseptic Transfer). 4) While holding the loop between the thumb and forefinger, grasp the mixed bacterial culture in …

  Exercise, Plate, Technique, Aseptic, Aseptic technique, Starke, Technique streak plate

SELECTIVE AND DIFFERENTIAL MEDIA

crcooper01.people.ysu.edu

as glucose-salts, as well as rich, chemically complex media such as brain heart infusion agar and tryptic soy broth. However, these general growth media may permit the growth unwanted microorganisms from clinical or environmental samples that are not relevant to the purpose of the microbiological examination.

  Agar, Selective, Differential

Biology of Fungi, Lecture 3: Fungal Structure and …

crcooper01.people.ysu.edu

Biology of Fungi Lecture 3: Fungal Structure and Function Page 2 of 5 Fungal Cell Wall u Functions Q Structural barrier Q Determines pattern of cell …

  Structure, Functions, Cells, Structure and function, Fungi, Structure and

DETERMINATION OF MICROBIAL NUMBERS

crcooper01.people.ysu.edu

Determination of Microbial Numbers, Page 4 of 20 given volume. Discerning between live and dead cells requires that the cells be stained in a specific manner. The chamber portion of the hemocytometer incorporates a special counting grid (Fig. 3). This grid has specific dimensions in length and width, typically 3 mm x 3 mm, with each grid divided

  Determination, Microbial, Length

EFFECTS OF PH ON MICROBIAL GROWTH

crcooper01.people.ysu.edu

• Sabouraud dextrose agar culture (3-5 days old) of Saccharomyces kudriavzevii (ATCC 2601; formerly designated as Saccharomyces cerevisiae) [abbreviated as S. kudriavzevii] Materials • Sterile saline (5 ml) in standard test tube • Sterile bulb transfer pipettes • TSB (3 ml) in 13 x 100 mm tubes • TSB, pH 3.0, (3 ml) in 13 x 100 mm tubes

  Agar, Growth, Effect, Microbial, Dextrose, Dextrose agar, Effects of ph on microbial growth

SULFIDE-INDOLE-MOTILITY (SIM) TEST

crcooper01.people.ysu.edu

Finally, indole production is detected in SIM medium due to the presence of casein peptone, an ingredient rich in tryptophan. Organisms that possess the enzyme tryptophanase degrade tryptophan to generate indole, pyruvic acid, and ammonia. The latter two are used for a cell’s nutritional needs, whereas indole is not and accumulates in the medium.

  Lodine

CARBOHYDRATE FERMENTATION TEST - Youngstown State …

crcooper01.people.ysu.edu

Microbiology Laboratory (BIOL 3702L) Page 1 of 6 CARBOHYDRATE FERMENTATION TEST Principle and Purpose Microorganisms need to generate energy in order to grow, divide, and survive. In any given environment, different sugars (i.e., carbohydrates) may be available. Microbes must possess the

  Microbiology, Boil, Fermentation

EFFECTS OF TEMPERATURE ON MICROBIAL GROWTH

crcooper01.people.ysu.edu

aureus, and Pseudomonas aeruginosa. The degree to which temperature affects the growth of these microbes shall be Figure 1. Growth rates of microbes as a function of temperature. Microbes can be differentiated based upon their ranges of temperature tolerance.

  Growth, Temperatures, Microbial, Microbial growth, Pseudomonas, Pseudomonas aeruginosa, Aeruginosa

Related documents

Lab Exercise 3: Media, incubation, and aseptic technique

faculty.sdmiramar.edu

Lab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. 2. Describe the different formats of media, plate, tube etc. 3. Explain how to sterilize it, and how to distribute it in different formats. 4. Produce Nutrient agar (NA) plates, NA slants, and Nutrient broths (NB)which will be used in

  Media, Exercise, Technique, Aseptic, Incubation, Lab exercise 3, And aseptic technique

18BTC103J-MICROBIOLOGY LABORATORY MANUAL

webstor.srmist.edu.in

3 Experiment No.1: Aseptic techniques and Media preparation Aim: To acquire the knowledge on aseptic techniques and develop the skill of media preparation for culturing the microbes Principle: Aseptic techniques are a fundamental and important laboratory skill in the field of microbiology.

  Media, Aseptic

BASIC CULTURE TECHNIQUE STREAK PLATE

crcooper01.people.ysu.edu

3) Using aseptic technique, sterilize a microbiological loop using either the gas burner or the Bacti-Cinerator (see the exercise entitled Basic Microbiology Technique: Aseptic Transfer). 4) While holding the loop between the thumb and forefinger, grasp the mixed bacterial culture in …

  Exercise, Plate, Technique, Aseptic, Aseptic technique, Starke, Technique streak plate

ISOLATION OF PURE CULTURES

fire.biol.wwu.edu

Exposure of sterile media to the environment will demonstrate the importance of aseptic technique. 1. Label two nutrient agar plates as "Exposure I" and "Exposure II." 2. Uncover the plate marked "Exposure I" and allow it to remain exposed in the lab for about 5 minutes. 3. Expose the plate marked "Exposure II" to a source of possible contaminants.

  Media, Technique, Aseptic, Aseptic technique

ANTIBIOTIC SENSITIVITY TESTING I. OBJECTIVES II. …

www.sas.upenn.edu

Gentamicin, 100 µg/ml, 3 ml 1/group Tetracycline HCl, 100 µg/ml, 3 ml 1/group Microwell plates (24 well) 2/group V. PROCEDURE (Students at each side of a table will form a group. Strict aseptic technique should be followed!) The Kirby-Bauer Disc Method 1. Obtain 2 plates and the cultures of E. coli and Staph. aureus. 2.

  Technique, Aseptic, Aseptic technique

Related search queries

Lab Exercise 3: Media, incubation, and aseptic technique, Media, Aseptic, TECHNIQUE STREAK PLATE, Aseptic technique, Exercise, Technique