Lab Exercise 3 Media Incubation And Aseptic Technique
Found 6 free book(s)Lab Exercise 3: Media, incubation, and aseptic technique
faculty.sdmiramar.eduLab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. 2. Describe the different formats of media, plate, tube etc. 3. Explain how to sterilize it, and how to distribute it in different formats. 4. Produce Nutrient agar (NA) plates, NA slants, and Nutrient broths (NB)which will be used in
18BTC103J-MICROBIOLOGY LABORATORY MANUAL
webstor.srmist.edu.in3 Experiment No.1: Aseptic techniques and Media preparation Aim: To acquire the knowledge on aseptic techniques and develop the skill of media preparation for culturing the microbes Principle: Aseptic techniques are a fundamental and important laboratory skill in the field of microbiology.
BASIC CULTURE TECHNIQUE STREAK PLATE
crcooper01.people.ysu.edu3) Using aseptic technique, sterilize a microbiological loop using either the gas burner or the Bacti-Cinerator (see the exercise entitled Basic Microbiology Technique: Aseptic Transfer). 4) While holding the loop between the thumb and forefinger, grasp the mixed bacterial culture in …
GELATIN HYDROLYSIS TEST
crcooper01.people.ysu.edu2) Using aseptic technique and a microbiology needle, inoculate the respective tube with the appropriate bacterium by stabbing the medium three-fourths of the way to the bottom of the tube. 3) Incubate all tubes for 1 to 7 days at 37°C. 4) Periodically throughout the incubation period, remove the tubes from the incubator and
ISOLATION OF PURE CULTURES
fire.biol.wwu.eduExposure of sterile media to the environment will demonstrate the importance of aseptic technique. 1. Label two nutrient agar plates as "Exposure I" and "Exposure II." 2. Uncover the plate marked "Exposure I" and allow it to remain exposed in the lab for about 5 minutes. 3. Expose the plate marked "Exposure II" to a source of possible contaminants.
ANTIBIOTIC SENSITIVITY TESTING I. OBJECTIVES II. …
www.sas.upenn.eduGentamicin, 100 µg/ml, 3 ml 1/group Tetracycline HCl, 100 µg/ml, 3 ml 1/group Microwell plates (24 well) 2/group V. PROCEDURE (Students at each side of a table will form a group. Strict aseptic technique should be followed!) The Kirby-Bauer Disc Method 1. Obtain 2 plates and the cultures of E. coli and Staph. aureus. 2.