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BASIC CULTURE TECHNIQUE STREAK PLATE

Copyright Chester R. Cooper, Jr. 2022 Microbiology Laboratory (BIOL 3702L) Page 1 of 6 BASIC CULTURE TECHNIQUE : STREAK PLATE Principle and Purpose The isolation of pure cultures of microorganisms is a TECHNIQUE essential to many types of experiments in microbiology as well as in the identification of potential pathogens. One very common way to isolate bacterial and other microbes is by employing the STREAK PLATE TECHNIQUE . In essence, the STREAK PLATE TECHNIQUE is a type of dilution scheme in which a single colony presumably arises from a single cell. In practice, a gradient of cells is established on the surface of an agar PLATE .

3) Using aseptic technique, sterilize a microbiological loop using either the gas burner or the Bacti-Cinerator (see the exercise entitled Basic Microbiology Technique: Aseptic Transfer). 4) While holding the loop between the thumb and forefinger, grasp the mixed bacterial culture in …

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crcooper01.people.ysu.edu

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crcooper01.people.ysu.edu

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