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Basic Principles of Cell Culture

1 Basic Principles of Cell CultureR. Ian FreshneyCentre for Oncology and Applied Pharmacology, Cancer Research UK BeatsonLaboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, Types of Cell Primary Explantation Versus Proliferation Versus Organotypic Substrates and Isolation of cells for Tissue Collection and Biosafety and Record Disaggregation and Primary Life Growth Serial Characterization and Microbial of of cells for Tissue Engineering, edited by Gordana Vunjak-Novakovic and R. Ian FreshneyCopyright 2006 John Wiley & Sons, 1. INTRODUCTIONThe bulk of the material presented in this book assumes background knowledgeof the Principles and Basic procedures of cell and tissue Culture .

purified cell strains from disaggregated tissue or primary culture or at first subcul-ture. These purified cell populations can then be combined in organotypic culture to recreate both the tissue cell density and, hopefully, the cell interactions and Basic Principles of Cell Culture 7

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