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Jurkat (ATCC number TIB-152) cell culture and formaldehyde ...

Cell Growth Protocol for Jurkat Cell Line From: HudsonAlpha/Caltech ENCODE group Date: 8/27/08 Prepared by: Norma Neff and Tim Reddy Jurkat (ATCC number TIB-152) cell culture and formaldehyde cross-linking Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts. The karyotype is pseudodiploid human male cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at cell culture protocol: Growth medium: Advanced RPMI 1640 (Gibco/Invitrogen) + 10% fetal bovine serum (Hyclone) + 10 mM Hepes + 100 units/ml penicillin + 100 g/ml streptomycin + 5% CO2 at 37oC.

Jurkat (ATCC number TIB-152) cell culture and formaldehyde cross-linking Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts. The karyotype is pseudodiploid human male cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%.

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