Transcription of Basic Principles of Chromatography - Anuraga Jayanegara
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Basic Principles of Chromatography - Anuraga Jayanegara
27c h a p t e rBasic Principlesof ChromatographyBaraem Ismail Department of Food Science and Nutrition, University of Minnesota,St. Paul, MN 55108-6099, Suzanne NielsenDepartment of Food Science, Purdue University,West Lafayette, IN 47907-2009, Batch Continuous Countercurrent Historical General Nielsen,Food Analysis,Food Science Texts Series, DOI ,c Springer Science+Business Media, LLC 2010473474 Part V Gas Liquid Paper Factors Column Supercritical Fluid Physicochemical Principles of Adsorption (Liquid Solid) Partition (Liquid Liquid) Coated Bonded Ion-Exchange Size-Exclusion Affinity Analysis of Chromatographic Separation and Developing a Column Qualitative Quantitative Study References498 Chapter 27 Basic Principles of INTRODUCTIONC hromatography has a great impact on all areas ofanalysis and, therefore, on the progress of sciencein general.
mobile phase are both liquid (partition chromatog-raphy, see Sect. 27.4.2 ). Paper generally serves as a support for the liquid stationary phase. The dis-solved sample is applied as a small spot or streak one half inch or more from the edge of a strip or square of filter paper (usually cellulose), which is then allowed to dry.
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