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Reverse-Phase High-Performance Liquid Chromatography ...

UC Berkeley College of ChemistryChemistry 105 Instrumental Methods in Analytical ChemistryReverse-Phase High-PerformanceLiquid Chromatography Analysis ofBiomoleculesShort ReportAuthor:JonathanMelvilleLab Partners:JakePrecht, PhuongTran, and JeremyHsuGraduate Student Instructors:RichardCooper& DanielMortensenApril 23, 20141 TheoryRP-HPLC, or Reverse-Phase High-Performance Liquid Chromatography is a type of chro-matography the features a Liquid mobile phase, higher resolving power than traditionalliquid Chromatography , and a specific combination of stationary- and mobile-phases thatresults in separations that are the opposite of a traditional HPLC. Chromatogra-phy, in general, utilizes the flow of a mobile-phase containing the analyte througha stationary-phase depending on the intermolecular forces of attraction between theindividual analytes and the stationary- and mobile-phases, the various components ofthe sample will exit the chromatographic column at different times[?]

utilized a polar stationary-phase and a nonpolar mobile-phase; \reverse-phase chromatog-raphy", by contrast, uses a nonpolar stationary-phase and a polar mobile-phase, resulting in an inverted separation from \normal-phase" chromatography: more polar analytes will

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  Chromatography, Chro matography, Raphy, Chromatog

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