Transcription of Bio-Plex Cytokine Assay
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Bio-Plex Cytokine Assay
Bio-Plex Cytokine AssayInstruction ManualFor technical service, call your local Bio-Rad office, or in the US, call 1-800-4 BIORAD (1-800-424-6723).For research use only. Not for diagnostic of ContentsSection 1 Introduction1 Section 2 Principle3 Section 3 Product Description4 Section 4 Materials Required or Recommended but Not Supplied5 Section 5 Sample Preparation and Premixed Standard Dilution8 Section 6 Assay Procedure for Premixed MultiplexPanels and Singleplex Assays14 Section 7 Mixing Multiplex Assays: Bead, Standard,and Detection Antibody Preparation21 Section 8 Bio-Plex Suspension Array System Operation27 Section 9 Data Analysis30 Section 10 Troubleshooting Guide31 Section 11 Safety Considerations36 Section 12 Publications Citing the Bio-Plex Cytokine Assay37 Section 13 Bio-Plex Multiplex Cytokine Assay Templateand Dilution Worksheet39 Section 14 Legal Notices42 Bio-Plex Cytokine Assay WorkflowAdd beadsAdd standardsAdd samplesIncubate/shake(30 min)*Add detectionantibodiesIncubate/shake(30 min)Add streptavidin-PEIncubate/shake(10 min)Resuspend beadsRead platePrewet filter platePrepare standards(30 min)Prepare samplesPrepare beadsPrepare detectionantibodyPrepare streptavidin-PEFilterFilter-wash 2xFilter-wash 3xFilter-wash 3xFilter-wash 3x* For the human Th1/Th2 magnetic panel, the incubation time is 60 1 IntroductionCytokines are important cell signaling proteins, media
Bio-Plex Cytokine Assay Workflow Add beads Add standards Add samples Incubate/shake (30 min)* Add detection antibodies Incubate/shake (30 …
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