Transcription of Buffer Formulations - Bio-Rad
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Buffer Formulations - Bio-Rad
Sample Preparation Buffers1 M Tris-HCl, pH (100 ml) Tris base g Deionized H2O (diH2O) 80 ml Adjust pH to with HCl diH2O to 100 M Tris-HCl, pH (100 ml) (catalog #161-0799) Tris base g diH2O ~60 ml Adjust to pH with HCl diH2O to 100 ml Store at 4 C10% SDS (10 ml) (catalog #161-0416) SDS g diH2O to 10 Bromophenol Blue (10 ml) (catalog #161-0404) Bromophenol blue 100 mg diH2O to 10 ml RIPA Solubilization Buffer (100 ml) 25 mM Tris-HCl pH , 150 mM NaCl, 5 mM EDTA, 1% NP-40 or 1% Triton X-100, 1% sodium deoxycholate, SDS NaCl g EDTA g NP-40 or Triton X-100 1 g Sodium deoxycholate 1 g SDS g diH2O 80 ml 1 M Tris-HCl, pH ml diH2O to 100 mlPhosphate Buffered Saline (PBS, 1 L) (w/v) sodium chloride in 10 mM phosphate Buffer , pH NaCl g KCl g Na2 HPO4 g KH2PO4 g diH2O 800 ml Adjust pH to with HCl or NaOH diH2O to 1 LSDS-PAGE Sample Buffer (2 , 8 ml) ()
Sample Preparation Buffers 1 M Tris-HCl, pH 7.6 (100 ml) Tris base 12.11 g Deionized H 2O (diH 2O) 80 ml Adjust pH to 7.6 with HCl diH …
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