Transcription of 蛋白质组学原理及应用案例 - CAS
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蛋白质组学原理及应用案例 - CAS
2019- 2000-2004 2004-2006 2006-2010 2011-2013 2013-2018 WB MRMHR/PRM VS LC-MS/MS Bottom-up proteomics or Shotgun proteomics MS2 @ .. LFQ TMT/iTRAQ SWATH .. @ MRMHR/PRM @ WB (Discovery proteomics ) --- LFQ/SWATH--- SILAC/ / 11.
⚫Unfortunately, the quality of quantitative data obtained via Western blotting is not comparable to that obtained with SRM. ⚫We posit that the request to validate quantitative MS data by Western blotting is no longer justified. ⚫In fact, it may be time to “turnthe tables”and request that Western blotting results be validated by MS.
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