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Cell Isolation Optimizing System - Worthington …

Worthington Biochemical CorporationTel: Fax: Isolation Optimizing SystemKit contains an assortment of the enzymes most frequenty used for tissue dissociation and primary cell Isolation applications and a Cell Isolation Guide which provides information on theory, techniques and lists hundreds of references according to tissue and at 2 - 8 CWorthington Biochemical Corporation Lakewood, New Jersey Fax: Isolation Optimizing SYSTEM1 Introduction ..1 Cell Isolation Theory Tissue Types ..2 Dissociating Enzymes ..2 Cell Isolation Techniques (using the kit) Methods and Materials ..5 Working with Enzymes Media Table Equilibration of Media Tituration Cell Harvesting Trypsinization Optimization Techniques ..7 General Guidelines Optimization Strategy Cell Quantitation Measure of ViabilityGeneral References ..10 Tissue Tables ..11-39 Collagenase Sampling Program ..40 Related Worthington Products ..41 IntroductionTissue dissociation/primary cell Isolation and cell harvesting are principal applications for enzymes in tissue culture research and cell biology studies.

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