Transcription of Chapter 10 Upstream Processing - Biomanufacturing
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Chapter 10 Upstream Processing - Biomanufacturing
2016 Montgomery County Community College Chapter 10 Upstream Processing Objectives 380 Chapter 10 - Upstream Processing This Chapter provides an overview of the key elements for operation of a Biomanufacturing Upstream Processing production area. Upon completion of this Chapter the student will be able to: distinguish between mammalian and microbial Upstream Processing describe the contribution of the areas of dispensing, media preparation, and fermentation/cell culture to the Upstream process as a whole describe proper methods to avoid cross-contamination in the dispensary, such as the use of airflow booths and following cleaning protocol explain the in-process controls for assessing media components illustrate the major types of bioreactors and associated instruments used for Upstream Biomanufacturing evaluate situations in or
382 Chapter 10 - Upstream Processing . Feed stream: consists of cells and culture media; the feed stream is cooled and microfiltered at a temp of 4 degrees Celsius . Fermentation: biopharmaceutical fermentation involves the culture of yeast or microbial cells for the purposes of protein drug production; most fermentations are aerobic; this is
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