Transcription of Chapter 10 Upstream Processing - Biomanufacturing
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Chapter 10 Upstream Processing - Biomanufacturing
2016 Montgomery County Community College Chapter 10 Upstream Processing Objectives 380 Chapter 10 - Upstream Processing This Chapter provides an overview of the key elements for operation of a Biomanufacturing Upstream Processing production area. Upon completion of this Chapter the student will be able to: distinguish between mammalian and microbial Upstream Processing describe the contribution of the areas of dispensing, media preparation, and fermentation/cell culture to the Upstream process as a whole describe proper methods to avoid cross-contamination in the dispensary, such as the use of airflow booths and following cleaning protocol explain the in-process controls for assessing media components illustrate the major types of bioreactors and associated instruments used for Upstream Biomanufacturing evaluate situations in order to make appropriate aseptic decisions obtain and interpret various assay values, apply the data.
382 Chapter 10 - Upstream Processing . Feed stream: consists of cells and culture media; the feed stream is cooled and microfiltered at a temp of 4 degrees Celsius . Fermentation: biopharmaceutical fermentation involves the culture of yeast or microbial cells for the purposes of protein drug production; most fermentations are aerobic; this is
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