Chapter 4 –Microscopy - Quantitative Light Imaging ...

Chapter 4 microscopy Gabriel PopescuUniversity of Illinois at Urbana ChampaignypgBeckman InstituteQuantitativeLightImagingLaborat oryElectrical and Computer Engineering, UIUCP rinciples of Optical ImagingQuantitative Light Imaging 460 Optical Imaging The Microscope can be approximated by 2 lenses [ [ [ , ]]] Resolution of Optical MicroscopespppyTube lensSampleF2 F2 Objective[ [ [ , ]]]yU(x,y)F 1[[ ]]FUxy[[[ ]]] []xyFFUU sign means inverted image Theobjectiveisthemostimportantpartofthem icroscope1[[,]]FUxy21[[[,]]][,]yFFUxyUMM The objective is the most important part of the microscope Usually a third lens (ocular) images F2 at , such that we can visualize it with the relaxed 4: microscopy 460 Optical Imaging The objective lens dictates the resolution or size of the Resolution of Optical Microscopesjsmallest object that the microscope can resolve.

4.5 PhaseContrast Microscopy ECE 460 –Optical Imaging Note: For a = 0 recover Dark Field Microscopy Assume“small”phaseshift Assume small phase shift cos 1; 2 2 2 2 (, ) 2 sin sin 2(,)sin Ixy a a aaxy PC couples into intensity

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  Light, Microscopy

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