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Microscopy I Light and Electron Microscopy

Microscopy ILight and Electron MicroscopyReplica of van Leeuwenhoek s(1632-1723) microscope constructed c. 1670. Moody Medical Library, Univ. Texas Medical Branch, Galveston, TXUse the information in this tutorial to supplement the visuals in lab and the information in Chapters 1, 8 and 9 in your lab manual .Replica of Culpepper tripod microscope built c. 1725 by Edmund Culpepper (1670-1738). Collection of Moody Medical Library, Univ. Tex., Galveston, TX. (Replica by Replica Rara Ltd. Antique Microscopes)ContentsI. Types of MicroscopyA. Light Microscopy1. Brightfield2. Darkfield3. Phase Contrast4. Polarizing5. DIC6. laser scanningB. Electron Microscopy1. Scanning2. TransmissionII. Specimen PreparationA. Light MicroscopyB. Scanning Electron MicroscopyC.

Light Microscopy Bright field Microscopes--the most common general use microscopes. Bright field microscopes are named because the microscopic “field” is bright, while the object being viewed is dark. - Simple design - Light directed at specimen is absorbed to form image

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