Transcription of Chapter 5 - Bacteriology - FWS
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Chapter 5 - Bacteriology - FWS
NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 1 Chapter 5 Bacteriology Jason Woodland USFWS - Pinetop Fish Health Center Pinetop, Arizona NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 2 I. Introduction This section defines the procedures and techniques used to correctly identify the target bacterial pathogens identified for the Survey (Yersinia ruckeri, Aeromonas salmonicida and Edwardsiella ictaluri). Proper identification relies on bacterial growth characteristics, appropriate biochemical tests, and corroboration by serological techniques. Pathogens of Regional Importance (PRIs) include: Citrobacter freundii, Edwardsiella tarda, Flavobacterium columnare and Flavobacterium psychrophilum. The later two bacteria have special requirements for culture and serological confirmation. Several excellent sources are listed in the Reference section for identification of PRIs and other bacteria that may be isolated from fish sampled for the Survey.
Salicin, Sorbitol, and Sucrose (saccharose). The procedures to be followed for each of these media are identical. 1. Inoculate carbohydrate tube (Appendix A “Carbohydrate Utilization media”) with growth from an 18 to 24 hour pure culture.
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