Transcription of CHAPTER 7 Identification and Characterization of …
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CHAPTER 7 Identification and Characterization of …
1 CHAPTER 7 Identification and Characterization of neisseria meningitidis N. meningitidis are gram-negative, coffee-bean shaped diplococci that may occur intracellularly or extracellularly in PMN leukocytes. N. meningitidis is a fastidious organism, which grows best at 35-37 C with ~5% CO2 (or in a candle-jar). It can grow on both a blood agar plate (BAP) and a chocolate agar plate (CAP). Colonies of N. meningitidis are grey and unpigmented on a BAP and appear round, smooth, moist, glistening, and convex, with a clearly defined edge. N. meningitidis appear as large, colorless-to-grey, opaque colonies on a CAP. Prior to Identification and Characterization testing procedures, isolates should always be inspected for purity of growth and a single colony should be re-streaked, when necessary, to obtain a pure culture.
1 CHAPTER 7 Identification and Characterization of Neisseria meningitidis. N. meningitidis are gram-negative, coffee-bean shaped diplococci that may occur intracellularly or extracellularly in PMN leukocytes. N. meningitidis is a fastidious organism, which grows best at …
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