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CHEM 344 Thin Layer Chromatography

CHEM 344 Thin Layer Chromatography Thin Layer Chromatography (TLC) is a useful technique for the separation and identification of compounds in mixtures. TLC is used routinely to follow the progress of reactions by monitoring the consumption of starting materials and the appearance of products. Commercial applications of TLC include the analysis of urine for evidence of "doping", the analysis of drugs to establish purity or identity of the components, and analysis of foods to determine the presence of contaminants such as pesticides.

scraping the adsorbent off the plate (or cutting out the spots if the supporting material can be cut) and extracting the substance from the adsorbent. Several factors determine the efficiency of a chromatographic separation. The adsorbent should show a maximum of selectivity toward the substances being separated so that the differences in

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