Transcription of Comparison of DNA sequencing, PCR-SSCP and PhaB assays ...
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Comparison of DNA sequencing, PCR-SSCP and PhaB assays ...
INT J TUBERC LUNG DIS 7(7):652 659. 2003 IUATLD. Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis C. Mani, N. Selvakumar, V. Kumar, S. Narayanan, P. R. Narayanan Tuberculosis Research Centre, Chetput, Chennai, India SUMMARY. SETTING: Tuberculosis Research Centre, Chennai, India. RESULTS A N D C O N C L U S I O N : The results obtained OBJECTIVE: To rapidly identify multidrug-resistant using the three assays were compared with those from Mycobacterium tuberculosis using phenotypic and geno- the conventional indirect sensitivity test.
Rapid methods for detection of rifampicin resistance653 Sanger’s method of dideoxy sequencing uses dideoxy nucleotides to allow chain termination at each base;
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