Transcription of DirectPCR Lysis Reagent (Cell) Cat # 301-C, 302-C
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DirectPCR Lysis Reagent (Cell) Cat # 301-C, 302-C
Viagen Biotech, Inc. 3530 Wilshire Blvd. #695 Tel: 800-649-3764; Fax: 800-649-3785 Los Angeles, CA 90010 Email: Revised February 2007 Store at 4 C Description: Certain compounds in animal tissues and cells inhibit PCR reactions. DirectPCR Lysis Reagents (Patent Pending) contain inhibitors of these PCR inhibitors. Therefore, DNA released in DirectPCR reagents is compatible for one-step PCR genotyping. DirectPCR Lysis Reagent (Cell) Cat # 301-C, 302-C 1. Suspend cells from a 10 cm plate in 200 300 l DirectPCR Lysis Reagent (Cell) containing freshly prepared mg/ml Proteinase K (Sigma, cat # p6556, not included). Proteinase K is stable in DirectPCR reagents for ~24 hrs. If a small number of cells are processed, and therefore it is difficult to weigh Proteinase K powder, use genomic PCR-quality Proteinase K solution (Viagen, cat #501-PK) at mg/ml (25-50 l Proteinase K solution per 1 ml DirectPCR Reagent ).
Viagen Biotech, Inc. http://www.viagenbiotech.com/ 3530 Wilshire Blvd. #695 Tel: 800-649-3764; Fax: 800-649-3785
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