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DNA Extraction using Qiagen DNeasy Mini Prep Kit

DNA Extraction using Qiagen DNeasy Mini Prep Kit protocol : Pretreatment for Gram-Positive Bacteria This protocol is designed for purification of total DNA from Gram-positive bacteria. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before DNA purification. 1. Harvest cells (maximum 2 x 109 cells) in a microcentrifuge tube by centrifuging for 10 min at 5000 x g (7500 rpm). Discard supernatant. 2. Resuspend bacterial pellet in 180 l enzymatic lysis buffer. 3. Incubate for at least 30 min at 37 C. After incubation, heat the heating block or water bath to 56 C if it is to be used for the incubation in step 5. 4. Add 25 l proteinase K and 200 l Buffer AL.

This protocol is designed for purification of total DNA from Gram-positive bacteria. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls ... dilution. Agarose gel electrophrosesis & Interpretation . Introduction

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