Transcription of DNA Fingerprinting
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DNA Fingerprinting
1 DNA Fingerprinting Unless they are identical twins, individuals haveunique DNA DNA Fingerprinting The name used for the unambiguous identifyingtechnique that takes advantage of differences in DNAsequence The process of DNA Fingerprinting begins byisolating DNA from blood, semen, vaginal fluids, hair roots, skin, skeletalremains, or elsewhere2 polymerase Chain Reaction (PCR) If there is only a small amount of DNA available for DNAF ingerprinting augment the amount of DNA using a technique called PCR PCR is doing DNA replication in a test tube3 polymerase Chain Reaction (PCR)Template Like ALL DNApolymerases Taq polymerase canonly add to the 3 endof an existingnucleotide A DNA primer that iscomplementary to thetemplate is used tosupply that 3 endand cool to anneal5 3 3 5 PrimerPrimerTemplate4 DNA Fingerprinting After we isolate the DNA and amplify it with PCR Treat the DNA with restriction enzymes cut DNA at specific sequences Everyone s DNA is different, so everyone s DNA will cut at differentsites This results in different sized fragments The different sized fragments are called restrictionfragment length polymorphisms, or RFLPs We can observe the different sized fragments in anexperiment that separates DNA based on fragment sizecalled Gel Electro
Polymerase Chain Reaction (PCR) Template ¥ Like ALL DNA polymerases ¥ Taq polymerase can only add to the 3 Õ end of an existing nucleotide ¥A DNA primer that is complementary to the template is used to supply that 3 Õ end and cool to anneal 5Õ 3Õ 3Õ 5Õ Primer Primer Template
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