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制限酵素を用いたプラスミド DNA の切断→Ligation …

DNA Ligation Transformation DNA pMYs(vector) 150ng/ul luc COP GFP Neo(insert) 100ng/ul Solution total250ul pMYs DNA Solution 133ul DNA20ug R4 Buffer 25ul total 1/10 XhoI 5ul DW 87ul ---------------------------------------- total 250ul luc COP GFP Neo DNA Solution 200ul DNA20ug R4 Buffer 25ul total 1/10 XhoI 5ul DW 20ul -------------------------------------- total 250ul 1.

1本振ったらブロックに戻し、もう1本を振るというように手早くやる。 ⑧1ml程度のGB-gel Solutionが2本ずつ出来るので、1回目700ul、2回目300ulという ように2回に分けてカラムを通し、全量を4℃、15,000rpmで遠心する（時間は1分弱、

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