Transcription of Double in situ hybridisation protocols - UCL
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Double in situ hybridisation protocols - UCL
In situ hybridization protocols By Pringle and W. D. Richardson Wolfson Institute for Biomedical Research and Biology Department University College London Gower Street London WC1E 6BT Tel 02076796724 or 02076796736 e-mail In situ hybridization showing labelling of individual cells expressing Fibroblast growth factor receptor 3 (Fgfr3) in an adult mouse brain coronal section developed with NBT/BCIP. Table of Contents OVERVIEW OF POTENTIAL 3 1. 3 2. TISSUE 4 3. HYBRIDIZATION 4 4. CORRECT PH .. 4 MINIMAL 5 IN VITRO TRANSCRIPTION OF LABELED 6 DIG labeling mixture for in 7 TISSUE 9 CRYOSECTIONING .. 11 KNIFE 11 CUTTING FROZEN 12 WRINKLE-FREE FROZEN IN SITU DETECTION METHODS, CHROMOGENIC AND 14 1. NBT/BCIP. NITROBLUE TETRAZOLIUM SALT + 1. 14 2. INT/BCIP P-IODONITROTETRAZOLIUM VIOLET + NITROBLUE TETRAZOLIUM 14 3. FAST 14 4. FLUORESCENT 14 IMMUNOHISTOCHEMISTRY AFTER IN SITU 15 SINGLE AND Double IN SITU HYBRIDIZATION WITH DIG AND/OR FITC-LABELLED 16 Hybridization 16 10x "salts".
In situ hybridization protocols By N.P. Pringle and W. D. Richardson Wolfson Institute for Biomedical Research and Biology Department University College London
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