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Evaluation of a Droplet Digital Polymerase Chain Reaction ...
Evaluation of a Droplet Digital Polymerase Chain Reaction Formatfor DNA Copy Number QuantificationLeonardo B. Pinheiro,*, Victoria A. Coleman, Christopher M. Hindson, Jan Herrmann, Benjamin J. Hindson, Somanath Bhat, and Kerry R. Emslie National Measurement Institute, Lindfield, New South Wales, Australia Bio-Rad Laboratories, Inc., Pleasanton, California, United States*SSupporting InformationABSTRACT: Droplet Digital Polymerase Chain Reaction (ddPCR) is a newtechnology that was recently commercialized to enable the precisequantification of target nucleic acids in a sample. ddPCR measures absolutequantities by counting nucleic acid molecules encapsulated in discrete,volumetrically defined, water-in-oil Droplet partitions. This novel ddPCRformat offers a simple workflow capable of generating highly stable partitioningof DNA molecules.
ABSTRACT: Droplet digital polymerase chain reaction (ddPCR) is a new technology that was recently commercialized to enable the precise quantification of target nucleic acids in a sample. ddPCR measures absolute
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