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Flow Cytometry Basics Guide - Bio-Rad

Flow CytometryPractical Advice to Get You Started in FlowFlow Cytometry Basics GuideVisit for more information Flow Cytometry Basics Guide | 1 Table of ContentsChapter 1 Principles of the Flow CytometerFluidics System ..3 Optics and Detection ..4 Signal and Pulse Processing ..6 Electrostatic Cell Sorting ..9 Chapter 2 Principles of FluorescenceFluorophores and Light ..11 Fluorescence ..12 Why Use a Fluorescent Marker? ..13 Which Fluorophores are Useful for Flow Cytometry ? ..13 Single and Tandem Dyes ..14 Fluorescent Proteins ..14 Fluorescence Compensation ..16 Compensation Controls ..18 Chapter 3 Data AnalysisGates and Regions ..19 Single-Parameter or Univariate Histograms.

laser beam or beams and the signal generated at each point in the cell’s path . These pulses can be mapped by plotting signal as a function of time . As the particle enters the laser beam spot, it will generate scattered light and fluorescence signals, which will ultimately manifest in a stream of electrons (current) from the anode of the PMT .

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